Polyclonal Antibody

Polyclonal Antibody

How to choose the antibody we need

How to choose the antibody we need,The first thing that should be considered is the custom. What experiment is used in, how much is the required amount? For scientific users, the antibodies are often used in the experiments WesternBlotting, IP, have certain

requirements for the specificity of the antibody, but not particularly stressed, often study a lot of new protein does not determine whether the new protein is very important, whether it will be the focus

of the future for a long period of time, so the amount of antibody is not large for scientific users in the customization of the new protein antibody, we strongly recommend that first used for experimental preparation of multi-resistant when the ideal reconsider the experimental results and the need for long term use of the antibody preparation of monoclonal antibody. Of course, if the specific requirements of

this antibody is particularly high (multi resistant specificity has been unable to meet the experimental requirements, such as used in flow cytometry, etc.), or should the custom monoclonal antibody.For industrial users, the antibody is often applied to the detection of quantitative experiments or functional experiments, is very high for the specificity of the antibody. Determine the target protein is basically known considerable commercial development value, and the product, once formed, the amount will be very big for industrial users, basically direct custom monoclonal antibody.The selected polypeptide or protein as an antigen? Decisions need to the antibody applied what experiments,

and that you can get the material to the experimental process, the protein in its natural state, recognized by antibodies is generally used in this case the protein conformational epitopes and linear epitopes. Such experiments are mainly CO IP, FACS, Neutralizing / Blocking ELISA assay such as natural proteins.

Protein is denatured during the experiment or the semi denatured state, when most of the conformational

epitope has been destroyed, are generally linear epitopes recognized by antibodies; this experiment Western, IHC / ICC / IF polypeptide as the antibody of the antigen preparation, the identification of linear epitope, protein is denatured or semi denatured state when in use, the result can be satisfactory.

But can not be guaranteed as the antibody of the antigen preparation protein recognize the native protein, recognition protein, both linear and conformational epitopes, substantially all of the experiments can be

applied, with the protein as an antigen should be preferred, but in the polypeptide antigen the case to meet the test requirements but obtained have a certain degree of difficulty in the protein, the polypeptide as

an antigen to prepare antibodies is more appropriate. www.cusabio.com/pro.php?bigid=11