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Chine (Rép. pop)

Médical

25-OH Vitamin D

Quantité : 10 grams

Vitamin D is converted to 25 hydroxy d vitamin (25ohd vitamin d) by hydroxylation in the liver and the active 1, 25-dihydroxy vitamin D in the kidneys.

25 hydroxyvitamin D is the main form of vitamin D in the body.

The level of serum 25 oh d vitamin d can reflect the level of vitamin D storage and correlate with the clinical symptoms of vitamin D deficiency

Similar to other steroid hormones, 1, 25 oh vitamin d3 regulates tissue cell differentiation and related gene expression, mainly regulating blood calcium level and affecting cell differentiation.

25-OH-VD has a high content in blood and a long half-life.

It is stable and is not directly affected by diet and lifestyle. Therefore, clinical measurement of 25 oh vitamin d can reflect the proper level of vitamin D in vivo.

The performance date of 25-OH-VD Rapid Quantitative Test Kit (FIA)

Manufacturer	SEKBIO
Parameter	25-OH-VD Rapid Quantitative Test Kit (FIA)
Linearity	5~100ng/mL
Detection limit	5ng/mL
Benchmark product	Roche

Method Comparison

Sample	Roche	Sekbio	Sample	Roche	Sekbio
1	78.02	90.71	31	65.73	66.38
2	91.61	78.97	32	30.42	34.37
3	55.17	45.84	33	20.96	23.14
4	79.13	89.79	34	97.97	84.54
5	25.62	24.29	35	59.41	60.24
6	26.72	22.68	36	7.14	6.97
7	6.43	6.38	37	41.47	35.85
8	20.47	19.58	38	18.83	18.69
9	39.42	43.85	39	19.34	17.11
10	63.00	66.96	40	20.08	18.41
11	16.17	16.50	41	56.48	57.70
12	15.02	16.24	42	56.90	59.08
13	34.91	31.64	43	14.04	13.45
14	9.11	9.57	44	80.34	88.07
15	88.37	94.53	45	78.14	88.27
16	94.91	91.89	46	20.08	19.09
17	88.62	92.17	47	32.51	36.18
18	69.36	66.03	48	76.21	69.40
19	55.04	61.92	49	15.30	15.31
20	7.78	7.25	50	5.77	5.69
21	69.54	69.22	51	18.25	17.30
22	65.46	70.73	52	84.60	97.40
23	48.07	44.21	53	14.87	16.25
24	24.31	26.41	54	28.87	24.48
25	7.40	6.41	55	68.35	72.81
26	63.97	57.94	56	42.60	42.17
27	42.03	43.31	57	15.03	17.40
28	77.15	72.94	58	17.84	18.93
29	13.38	11.32	59	11.06	9.91
30	51.65	59.58	60	84.63	78.25

Linear

Cal	Concentration	Result	AVERAGE
S1	4.76	4.80	4.86
		5.19
		4.59
S2	14.78	15.75	14.98
		13.18
		16.01
S3	31.67	34.72	33.01
		33.23
		31.07
S4	60.84	62.68	60.91
		58.22
		61.83
S5	81.56	78.10	76.20
		77.02
		73.48
S6	103.58	109.51	113.18
		123.39
		106.63

Accuracy

Cal	concentration	Result	Bias
A1	14.78	15.47	4.6%
		15.58	5.4%
		16.22	9.7%
A2	31.67	32.70	3.3%
		29.35	-7.3%
		29.57	-6.6%
A3	60.84	57.26	-5.9%
		63.46	4.3%
		63.46	4.3%

Precision

Cal	Concentration	Cal	Concentration	Cal	Concentration
R1	14.19	R2	35.21	R3	59.28
	14.04		27.37		61.50
	16.50		30.93		54.36
	15.71		32.58		61.36
	15.22		30.98		52.39
	16.90		28.45		68.70
	13.10		36.27		63.53
	15.57		31.41		54.62
	14.81		27.06		62.28
	13.54		34.88		57.39
AVE	14.96	AVE	31.51	AVE	59.54
SD	1.25	SD	3.26	SD	4.96
CV	8.4%	CV	10.4%	CV	8.3%

Vitamin D Products

Antibody	Application
Sheep Anti-human 25-OH Vitamin D mAb	For immunodiagnostic: ELISA, LFA, CLIA
Mouse Anti-human 25-OH Vitamin D mAb
Humanized Anti-human 25-OH Vitamin D mAb

VD product Introduction

Optimal Vitamin D status is determined by measuring the metabolite 25-OH vitamin D in the bloodstream. This form of vitamin D serves as the primary storage compound in the human body, significantly outweighing the biologically inactive circulating 1,25 (OH)2 vitamin D by approximately 1000-fold.

The circulating half-life of 25-OH vitamin D is approximately 2-3 weeks. It's worth noting that over 95% of measurable 25 0h vitamin d in the serum consists of 25-OH vitamin D3, while 25ohd2 becomes detectable only in individuals who supplement with vitamin D2.

Vitamin D-binding protein

The human GC is approximately 58 kDa in size and consists of 458 amino acids. Its genetic code is comprised of 1690 nucleotides located on chromosome 4 (4q11–q13). A notable feature of GC is its primary structure, which includes 28 cysteine residues forming multiple disulfide bonds. The protein is divided into three domains: domain 1 comprising 10 alpha helices, domain 2 consisting of 9 helices, and domain 3 with 4 helices.

Interestingly, the metabolism of these domains appears to differ, particularly in their interaction with vitamin D. Research indicates that the vitamin D binding protein, a component of GC, may exhibit a higher affinity for 25(OH)D3 (the metabolite of vitamin D3) compared to 25(OH)D2 (the metabolite of vitamin D2). This finding was observed in a specific study.

Six point calibration test results

Conc.ng/ml	RLU		AVER	CV	S/N
0.00	1988864	1989951	1989408	0.04%	1.00
30.00	1701638	1717603	1709621	0.66%	1.16
75.00	1199394	1201402	1200398	0.12%	1.66
150.00	680993	677204	679099	0.39%	<span s

Localisation : B503, Building 1, Phase II, Yinxing Science and Technology Park, Guanlan, Shenzhen, China, 518110 Shenzhen,
Personne à contacter : LUO MIKE, 0755 2101 6139

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Site web : www.sekbio.com/

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25-OH Vitamin D

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