About The Reason And Solution Of The HPLC FAQs
1. Why does the retention time sometimes drift and sometimes change rapidly when analyzed by HPLC? How to solve it?
About drift
① The temperature control is not good. The solution is to use a constant temperature device to keep the HPLC column temperature constant.
② The mobile phase changes and the solution is to prevent the mobile phase from evaporating, reacting, etc.
③ The column is not well balanced, and the column needs to be equilibrated for a longer time.
About rapid change
① The flow rate changes. The solution is to reset the flow rate to keep it stable.
② There are air bubbles in the pump. The air bubbles can be expelled by exhaust operation.
③ The mobile phase is inappropriate. The solution is to change the mobile phase or make the mobile phase properly mixed in the control room.
2.Main reasons and solutions for insufficient HPLC sensitivity
① Insufficient sample size, the solution is to increase the sample size.
② The sample does not flow out of the column. The mobile phase or column can be changed depending on the chemistry of the sample.
③ The sample does not match the detector. Adjust wavelength or change detector based on sample chemistry.
④ Too much attenuation. Just adjust the attenuation.
⑤ The time constant of the detector is too large. The solution is to reduce the time parameter.
⑥ Detector's pool window is polluted. The solution is to clean the pool windows.
⑦ There are air bubbles in the test cell. The solution is the exhaust.
⑧ Improper pressure measurement range of the recorder, just adjust the voltage range.
⑨ The mobile phase flow rate is not suitable, just adjust the flow rate.
⑩ The detector and recorder exceed the calibration curve. The solution is to check the recorder and detector and redo the calibration curve.
About The Reason And Solution Of The HPLC FAQs
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