Factors Affecting the Performance of C18 HPLC Columns
Physical properties of the chromatographic column could affect the performance of C18 HPLC columns.
Performance factors of C18 HPLC columns:
Silica gel purity
Class A silica gel has high residual silica hydroxyl with a negative charge and high metal content on the acidic surface, which leads to trailing of alkaline compounds.
Due to the low metal content and high silica hydroxyl pKa of class B silica gel, tail dragging of alkaline compounds does not occur.
Performance factors of C18 HPLC columns:
Column size
Short column – short running time, low column pressure
Long column – high resolution, long running time
Narrow diameter column – detector has high sensitivity
Performance factors of C18 HPLC columns:
Particle shape and size
When the mobile phase with high viscosity is used, the spherical particles can reduce the column pressure and prolong the life of the column. Smaller particles have higher column efficiency but can cause column pressure to be too high.
Performance factors of C18 HPLC columns:
Surface area and aperture
The high surface area has strong retention capacity, column capacity and separation degree for separating multi-component samples. Low surface area fillers usually reach equilibrium quickly and are particularly important for gradient leaching.
Personne à contacter : Bruce Lu, +86 29 89284429
Bonne affaire : acheter au vendeur
Nous vous invitons à lire nos conditions générales d'utilisations. Vous pouvez aussi vous rendre sur nos FAQ et consulter notre page d'informations sur les risques liés à la contrefaçon.
Cette page concerne les importateurs et exportateurs de Factors Affecting the Performance of C18 HPLC Columns Rechercher dans la catégorie : Divers Rechercher dans la catégorie : hplc, factors, performance, columns, affecting |
Mardi 09 juin 2015
Quantité : 5000 - Prix : 11-15
je suis a la recherche d'un grossite pour vendre des pantalons jeans avec une excellente qualite et un bon prix Localisation : 19 RUE NAPOULSE TUNIS, 1002 TUNIS, Personne à contacter : Sofiene fathallah, +21 6 20 77 91 43
Global+ de commerce SARL
- 1002 - TUNIS
- +21 6 20 77 91 43
Jeudi 01 septembre 2011
100% pure Argan soap, Excellent for dry or sensitive skin. Naturally high in unsaturated fatty acids, oleic and linoleic acids and Vitamin E.Great slightly nutty natural scent. *Price : 0.9 - 1.6 Euro/100grs , MOQ 1200 pieces (100kg)Localisation : Soc 16 Derb Bennis, Fès médina, Fez...
Al Hashimia Corporation
- alhashimiacorp
- - Fez
- 00212667315723
Vendredi 13 juin 2008
Quantité : As per dem - Prix : Negotiable
We have vast arrangement and well experienced team of workers for Leather Garmrnts-Fashion, Motor Bike Jacket,Pants, Skirts etc. High quality leather garments as per requirements of Clients. If some one wants us to develop his design,do send us design and we will feel pleasure to...
Sayor Int'l.
- sayor
- 51310 - Sialkot
- +92 5 23 61 05 15
- 0092 321 7101608