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Chine (Rép. pop)

Matières premières

Keratinase

Quantité : 5000tons Prix : 1-50USD/KG

Keratinase Products

Protease catalyzes peptide bond and hydrolyzes protein into peptone, hydrazone, polypeptide and amino acid. As a kind of alkaline serine protease, keratinase enzyme not only has the function of normal alkaline protease, but also can break down disulfide bond and disintegrate keratin.

Specification of Keratinase

Name	Keratinase	Product Series Name	NutriGen Keratinase
Activity	100000-200000 U/g
Strain	Parengyodontium album	EC code	EC 3.4.99.11
Form	Powder,Liquid	Carrier	Starch
Color	White, Light yellow	Loss on drying	≤ 10%
Working pH	6.0-12.5	Optimum pH	11
Working T	30℃-70℃	Optimum T	60℃
Particle size	80% pass 40 mesh
Package	25kg /bag
Storage	Stored 6 months in dark, cool and dry conditions
Caution	Avoid direct sunlight, high temperature

Definition and Dosage of Activity Keratinase

One unit (U) of protease activity is defined as the amount of enzyme china which liberates 1 μg tyrosine per minute from substrate casein at 40℃ and pH 10.5 under the conditions of the test.

Recommended dosage is 50-100g/t with 50000 U/g in complete feed.

Functions of Keratinase

1. Supplement the deficiency of endogenous protease for animal.

2. Increase the digestibility of protein and amino acid in poultry and swine feed ingredients.

3. Degrade the trysin inhibitor and lectin, this is what endogenous protease can't do.

4. Optimized the use of lower digestibility feed ingredient e.g corn, sorghum, feather meal,soybean meal and blood plasma powder.

5. Creat a better environment for animal and decrease pollution from nitrogen for human.

Application of Keratinase

1. The matrix value of keratinase (100g/t)

Nutrient	Poultry
Nutrient	Value	Amount provided in the diet
Metabolizable energy（Mcal/kg）	600	0.060
Crude protein, %	5400	0.540
Lysine, %	150	0.015
Methionine, %	180	0.018
Cystine, %	150	0.015
Threonine, %	210	0.021
Tryptophane, %	90	0.009
Isoleucine , %	150	0.015
Arginine, %	285	0.0285
Valine, %	255	0.0255

2. Application case: Improve the performance and digestibility of nutrients.

Experimental animal: 840 one-day-old AA broilers, 6×35 broilers/treatment.

Experimental design:

Table1. Experimental design

Item	Diet
PC	Basal diet
NC	Basal diet-0.5% crude protein
Group I	NC+125g/t competitor
Group II	NC+100g/t Keratinase(30000U/g)

Experimental cycle: 42 days

Results:

Table2 Effect of each treatment on the growth performance of AA broilers (g, %)

Items	PC	NC	Group I	Group II
Initial weight,g	62.79±0.31	62.58±0.29	62.56±0.33	62.33±0.27
1-21days
21stAG,g	986.16ab±10.08	977.41b±8.22	1013.59ab±25.72	997.17ab±10.53
ADFI,g	62.08± 1.43	60.93±1.10	62.09±2.04	58.71±2.12
ADG,g	43.94±0.47	43.45±0.39	45.12±1.29	44.33±0.53
F/G	1.41±0.03	1.40±0.03	1.38±0.05	1.33±0.04
Death rate,%	1.43±1.57	3.45±2.21	5.38±6.24	3.33±5.24
22-42days
22ndAG,g	1140.08±1.30	1135.33±5.97	1136.73±7.06	1138.33±5.41
42ndAG,g	2985.77±41.56	2971.06±47.61	3058.21±58.29	3010.25±44.17
ADFI,g	179.00±3.12	178.64±2.86	175.81±3.64	174.22±2.64
ADG,g	87.88±2.01	87.42±2.43	91.50±2.85	89.14±2.20
F/G	2.04±0.07	2.05±0.03	1.93±0.05	1.96±0.06
Death rate,%	2.00±2.19	1.33±2.07	0.67±1.63	1.33±2.07
1-42days
ADFI,g	120.51±2.39	119.78±1.63	118.94±2.01	116.47±1.60
ADG,g	69.59±0.98	69.25±1.13	71.32±1.39	70.18±1.05
F/G	1.73±0.02	1.73±0.02	1.67±0.02	1.66±0.03
Death rate,%	3.43±3.76	4.78±1.73	6.05±5.74	4.67±4.59

Note: In the same row, values with different small letter superscripts mean significant difference(P<0.05).</span>

Diagram1 Effect of keratinase on the nutrient digestibility of broilers

Table3 Effect of keratinase on the amino acid digestibility of broilers （%）

Items	PC	NC	Group I	Group II
Asp	55.87±4.44	52.59±2.57	54.52±8.03	57.52±5.76
Thr	63.09±7.47	61.47±5.32	62.17±5.74	63.69±5.19
Ser	69.11±5.51	65.44±4.61	65.80±7.04	68.04±4.10
Glu	69.31±3.41ab	64.68±1.35b	67.12±5.82	74.82±3.39a
Gly	55.36±15.14ab	42.03±4.21b	49.11±11.59b	67.00±1.89a
Ala	31.82±2.11b	34.51±4.37b	35.35±5.19	35.92±4.82b
Val	46.32±0.75	45.59±1.66	44.68±4.68	50.18±3.73
Met	72.53±1.35	70.69±2.65	75.17±3.79	74.29±3.31
Ile	46.45±0.53b	47.40±2.87ab	47.47±5.60	51.65±4.65ab
Leu	56.28±1.57	52.87±7.19	55.44±8.01	53.05±4.90
Tyr	50.93±3.67	47.91±4.34	50.94±10.97	56.55±8.06
Phe	66.11±2.37	61.58±1.25	62.81±7.79	68.80±6.93
His	31.90±4.72ab	25.09±7.33b	28.06±14.13	40.79±5.43ab
Lys	78.58±4.31ab	71.44±2.54b	74.47±8.35	82.45±1.20a
Arg	79.15±2.86ab	75.18±3.10b	75.80±5.99	80.68±3.36ab
Pro	67.76±0.73b	70.57±1.32ab	69.36±7.39	68.06±2.90b
TAA	62.69±0.43ab	59.17±1.39b	60.83±5.69	66.45±3.73a

Conclusion of Keratinase

On the basis of these results, it is concluded that the supplement of keratinase into a low CP diet was as effective as normal level in broiler. As shown in diagram 1, keratinase improve the digestibility of dry matter, crude protein, crude fat, general energy and apparent metabolic energy. And in table 4, keratinase has a positive effect on the amino acids digestibility. In this experiment, it is appeared that keratinase improve the growth performance of broiler by increase average diary gain and feed conversion rate.

Localisation : Rm 1107 Luneng International Center, 2666 Erhuan South Road, Shizhong District, Jinan, China, 250002 Jinan,
Personne à contacter : Xi Julia, 86 531 55772899

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